Services Offered

Product No. Name of Service Service Requirements Turnaround Time
DNA Sequencing Services
SS1001 Single Pass DNA Sequencing Customer to provide purified DNA templates with sequencing primer(s) in separate tubes. 1-2 working days
SS1002 Single Pass DNA Sequencing, 96-well format High Throughput (HT) DNA Sequencing. Leave well H12 empty for our control reaction, Each plate comprises of maximum 95 samples with normalized OD. 2-3 working days
SS1003 Single Pass DNA Sequencing with Quantification Similar to SS1001, except that an additional charge will be levied for agarose gel electrophoresis quantification. 1-2 working days
SS1005 Ready to Load Sequencing We accept reactions done using BigDye® v3.1. 1-2 working days
SS1006 Difficult Template Sequencing Refers to DNA templates with 70% GC-rich/ 70% AT-rich/ GT-rich/ repetitive sequences. 2-3 working days
SS1007 Bi-Directional DNA Sequencing Customer to provide purified DNA templates with sequencing primer(s) in separate tubes. 1 service comprises of 2 single pass sequencing reactions from the same DNA template. 1-2 working days
SS1008 Primer Walking of Constructs Service - Single Pass. Charge per bp of data. Sequencing runs bi-directionally until a length of overlapping sequences is obtained. Customer to provide vector name and RE cut sites (where applicable) for data alignment. Service inclusive of primer design, primer synthesis, DNA sequencing and data alignment.  1kb per week
SS1009 Primer Walking of Constructs Service - Bi-Directional. Charge per bp of data. Sequencing runs bi-directionally until the end of insert. Customer to provide vector name and RE cut sites (if applicable) for data alignment. Service inclusive of primer design, primer synthesis, sequencing and data alignment.  1kb perweek
SS1010 Direct Genomic Sequencing Customer to provide purified genomic DNA directly (without additional PCR) and its specific sequencing primer.The purity, amount of genomic DNA; and the priming efficiency of its sequencing primer are crucial to the outcome of the sequencing reaction. 2-3 working days
SS1011 BAC (Bacteria Artificial Chromosome) End Sequencing BAC holds large genomic DNA constructs, ranging from 150 to 350 kbp. Customer to provide purified BAC and its specific sequencing primer (where applicable). The purity and amount of BAC is crucial to the outcome of the sequencing reaction. 2-3 working days
SS1201 DNA Sequencing Service + Plus

Customer to provide unpurified PCR products. PCR clean-up will be performed prior sequencing. This service is recommended for unpurified PCR products that shows single band on agarose gel, but not applicable for difficult template type sample. By using proprietary PCR clean-up techniques that delivers the highest recovery, we guarantee longer read under sequencing plus service at > 1000 bases.

2-3 working days
Sample Preparation and Quantification Service
SS1012 DNA Sequencing Sample Preparation - PCR Clean-up PCR purification recovers all dsDNA and removes the excess PCR reagents and primers. This is only recommended for single band-product. 1-2 working days
SS1013 DNA Sequencing Sample Preparation - PCR Clean-up 96-well format High throughput PCR purification recovers all dsDNA and removes the excess PCR reagents and primers in 96-well plate. This is recommended only for single band-product. 
Price per sample for >= 80 samples in a 96-well plate.
2-3 working days
SS1014 DNA Sequencing Sample Preparation - Gel Extraction by General Agarose PCR purification by gel extraction using general agarose gel. Customer to indicate the fragment size to be excised. 1-2 working days
SS1015 DNA Sequencing Sample Preparation - Gel Extraction by General Agarose 96-well format High throughput PCR purification by gel extraction using general agarose gel. Customer to indicate the fragment size to be excised.Price per sample for >= 80 samples in a 96-well plate. 3-4 working days
SS1017 DNA Sequencing Sample Preparation - Plasmid Extraction Customer to provide colony plate prepared from single colony, vector name, antibiotic resistant and concentration of antibiotic. Fresh colony plate (not more than 2 weeks) or fresh broth (not more than 1 month) is recommended. 2-3 working days
SS1018 DNA Sequencing Sample Preparation - Plasmid Extraction 96-well format High throughput plasmid extraction in 96-well plate. Price per sample for >= 80 samples in a 96-well plate. 2-3 working days
SS1019 Ready to load Sequencing with Clean-up Customer to provide us with pre-cycled BigDye® sequencing reaction. We will perform the post-cycle clean-up to remove un-incorporated dyes before loading on our Genetic Analyzer. 1 working day
SS1020 DNA Sequencing Sample Quantification - By Agarose Gel This service allows customers to skip the hassle of gel electrophoresis to check purity and concentration of DNA template before cycle sequencing.  1 working day
SS1021 DNA Sequencing Sample Quantification - Spectrophotometer Reading This spectrophotometric analysis service determines DNA template concentration and ratio of 260/280 before cycle sequencing. 1 working day
SS1047 DNA Sequencing Sample Preparation Service 
- RNase Treatment for plasmid DNA, followed by DNA precipitation and purification prior sequencing
Purification service that is recommended for plasmid DNA that shows RNA contamination before cycle sequencing. 1-2 working days
DNA Sequencing Custom Services
SS1024 DNA Sequencing Service - Publication Quality We run the reactions twice to generate 2 different .ab1 files from the same DNA and its sequencing primer(s). Discounted price applies to the duplicate reaction. 1 working day
DNA Sequencing Design and Analysis Services
SS1026 DNA Sequencing Analysis - BLAST Blast-N (nucleotides) from NCBI 1 working day
SS1027 DNA Sequencing Analysis - Data Alignment with 2 sequences 1 alignment data is generated from 2 DNA sequencing results (usually forward and reverse sequencing). 1 working day
SS1028 DNA Sequencing Analysis - Data Alignment with 3 sequences 1 alignment data is generated from 3 DNA sequencing results (usually forward, reverse & internal sequencing). 1 working day
SS1029 DNA Sequencing Primer Design and Synthesis (Single primer) The sequencing primer is designed from 1 sequencing result. It usually apply for 1 directional sequencing, for the fragment size > 1.5 kbp. 3-4 working days
SS1030 DNA Sequencing Primer Design and Synthesis (Forward and Reverse primer) The sequencing primers are designed from 2 sequencing results. It usually applies to bi-directional sequencing, for the fragment size > 3.0 kbp. 3-4 working days
SS1042 DNA Sequencing Service - Dendrogram. Price per submission. 1 dendrogram will be created using the sequencing traces generated by our Genetic Analyzers. 1 working day
SS1043 DNA Sequencing Service - Dendrogram with previous data. Price per submission. Customer to provide other sequences to be included in this analysis. 1 dendrogram will be created using the provided sequences, which included the sequencing traces that not generated by our Genetic Analyzers. 1 working day
Other Add-on Services
SS1031 DNA Sequencing Results in CD up to 700MB (Price per CD) Soft copy of results in CD. Soft copy is provided free of charge for customers who simply want to download the results in our link. 1 working day
SS1032 Hardcopy Electropherogram Results (price per Reaction ID) Physical colour printout of electropherogram. (Electropherograms are available for print through electronic files sent as part of your results package, at no additional cost.) 1 working day
SS1033 Softcopy DNA Sequencing Report. Price per Order ID This sequencing report contains the data evaluation for each reaction according to the chemistry guide of BigDye® cycle sequencing by Applied Biosystems. 1 working day
SS1034 Storage of Primers per 2 months for each tube. Sequencing primers will be discarded after 1 week from the completed order. A nominal charge applies if you need us to keep them in our laboratory. 2 months
SS1036 DNA Sequencing Service - Drying of Ready to Load Samples. Price per Reaction. Residual ethanol on the precipitated BigDye® cycle sequencing will generate relatively low signal data. This service is available for the customers who are not able to dry the BigDye® reactions completely. 1 working day
SS1037 Both Softcopy and Hardcopy DNA Sequencing Report. Price per Order ID Softcopy of DNA sequencing report to be sent through result link. Coloured hardcopy DNA sequencing report will be delivered separately. 1 working day
SS1038 Storage of Samples per 2 months for each tube. All provided DNA sample(s) will be discarded after 1 week from order completion. A nominal charge applies if you wish for us to continue keeping them in our laboratory. 2 months
Expedite Service - Pickup Pick-up Charge for Expedite Service. One-time charge per order. Our Expedite Service is only available in selected locations. Please enquire if your location is covered.  Request through 1oo before 9am, sample ready for pick-up before 11am. T&C apply.
Seq Expedite Service-SP DNA Sequencing Expedite Service, Single Pass Reaction. Price per reaction. Our Expedited Service is only available in selected locations. It is limited to 95 reactions per order and only available through our online ordering system (1oo) Not applicable if samples require add-on services e.g. clean-up, gel extraction before sequencing. Same day, before 6pm.
T&C apply.
Seq Expedite Service-RTL DNA Sequencing Expedite Service, Ready-to-load. Price per load. Our Expedited Service is only available in selected locations. It is limited to 95 loadings per order and only available through our online ordering system (1oo). Not applicable if samples require add-on services e.g. clean-up before sequencing. Same day, before 6pm.
T&C apply