Reagents for Protein Electrophoresis

ExactPro Broad Range Prestained Protein Ladder (BIO-5150)

Prestained-Protein-Ladder.Description

Prestained Protein Ladder contains 13 prestained recombinant proteins that cover a wide range of molecular weights from 3.5kDa – 245kDa. An orange reference band of ~75kDa and a green reference band of ~25kDa provides easy monitoring of protein separation.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.

Storage Buffer

20mM Tris-H3PO4 (pH 7.5 @ 25°C) 
15% (v/v) Glycerol 
2% (w/v) SDS 
10mM DTT 
3.6mM Urea

Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel.

Recommendations 

- Thaw ladder at room temperature to dissolve precipitated solids. Do not boil.
- Mix solution gently to ensure that it is homogeneous.
- Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
     * 3µl per loading on 15-well mini-gel
     * 5µl per loading on 10-well mini-gel
     * 1.5 – 2.5µl per loading for general Western transferring
     * Double the loading volume for thicker or larger gel.

Remarks

  • Each lot of Prestained Protein Ladder is calibrated with unstained protein ladder and calculated apparent molecular weights are as the picture.
  • Prestained proteins can have different mobilities in various SDS-PAGE-buffer systesm. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system.
  • In 8% or 10% gels, low molecular weight proteins may migrate with the dye front.
  • Suitable for use in Western blotting with all common membranes.
  • Longer transfer times or higher transfer voltages may be required for Western blotting of proteins larger than 100kDa.

Product Information

Product No.

Description

Product Details

MSDS

BIO-5150-250ul

ExactPro Broad Range (10-245kDa) Prestained Protein Ladder, 250ul

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BIO-5150-500ul

ExactPro Broad Range (10-245kDa) Prestained Protein Ladder, 500ul

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See also BUF - Product Listing for our Listing of popular buffers for Protein Electrophoresis.