Reagents for DNA Electrophoresis

ExactMark 100bp DNA Ladder, Ready-To-Use (BIO-5130)

100bp-DNA-Ladder-Ready-To-Use

Description

Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of eleven individual DNA fragments (in base pairs): 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100. It contains two reference bands of 1500 and 500bp for easy orientation. Orange G and xylene cyanol FF are used as tracking dyes.

Storage Buffer

10mM Tris-HCl (pH 8.0) 
1mM EDTA

Protocol for Loading

- Load 5µl of the solution into a 5mm agarose gel lane.
- It is recommended to load 1μl (0.1μg) of DNA ladder per 1mm of gel lane.

 

Recommendations

- Do not heat solution prior to loading.
- Adjust sample concentration to be approximately equal to the amount of DNA in the nearest band of the ladder for quantification purpose.
- Visualize DNA by staining with DNA stain (C/No: BIO-5170-1ml).

ExactMark 1kb DNA Ladder, Ready-To-Use (BIO-5140)

1kb-DNA-LadderDescription

Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of thirteen individual DNA fragments (in base pairs): 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1000, 750, 500, and 250. It contains three reference bands of, 3000 and 1000bp for easy orientation. Bromophenol blue is used as tracking dye.

Storage Buffer

10mM Tris-HCl (pH 8.0) 
1mM EDTA

Protocol for Loading

- Load 5µl of the solution into a 5mm agarose gel lane.
- It is recommended to load 1μl (0.1μg) of DNA ladder per 1mm of gel lane.

 

Recommendations

- Do not heat solution prior to loading.
- Adjust sample concentration to be approximately equal to the amount of DNA in the nearest band of the ladder for quantification purpose.
- Visualize DNA by staining with DNA stain (C/No: BIO-5170-1ml).

Tri-Color 6X DNA Loading Dye (BIO-5160-6ml)

6X-DNA-Loading-DyeDescription

Used for loading DNA markers and samples on agarose or polyacrylamide gels. It contains three dyes – Orange G, Xylene cyanol FF and Bromophenol blue. This allows better visual tracking of DNA migration during electrophoresis.

6X DNA Loading Dye 

0.15% Orange G 36% Glycerol
0.03% Bromophenol blue 10Mm Tris-HCl (pH 7.4)
0.03% Xylene cyanol FF 50mM EDTA (pH 8.0)

Protocol for Loading

- Mix 1 part of dye solution with 5 parts of DNA sample.

FloroSafe DNA Stain, 1ml (BIO-5170-1ml)

DNA-StainDescription

Non-carcinogenic alternative of Ethidium Bromide, DNA Stain can be used as a safer alternative compared to the traditional ethidium bromide stain used for DNA and RNA detection in agarose gel. Compared to ethidium bromide, it has low toxicity and mutagenicity. DNA Stain has two secondary fluorescence excitation peaks (~270 nm; ~295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to Ethidium Bromide when bound to DNA which is at about 530 nm.

Usage Protocols

Precasting method:

- Prepare 100ml of 0.8-3.0% agarose gel solution.
- Heat till solution is completely clear.
- Add 4-6µl of DNA stain and mix gently with gel.
- Cool gel to 50-60°C and cast gel into gel tray.
- Load samples into solidified gel and perform electrophoresis.
- Detect bands under UV illuminator.

Post-staining method:

- For agarose gel less than 0.5cm thickness, 10 – 25µl of the stain should be used per 100ml of buffer
- Staining time is optimized between 5 – 60 minutes; amount of stain varies with gel thickness and concentration of agarose.

Product Information

Product No.

Description

No of Applications

Details

MSDS

BIO-5130-50ug

ExactMark 100bp DNA Ladder, Ready-To-Use, 50ug

~100 applications

Protocol

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BIO-5130-100ug

ExactMark 100bp DNA Ladder, Ready-To-Use, 100ug

~200 applications

Protocol

BIO-5140-50ug

ExactMark 1kb DNA Ladder, Ready-To-Use, 50ug

~100 applications

Protocol

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BIO-5140-100ug

ExactMark 1kb DNA Ladder, Ready-To-Use, 100ug

~200 applications

Protocol

BIO-5140-250ug

ExactMark 1kb DNA Ladder, Ready-To-Use, 250ug

~500 applications Protocol

BIO-5160-6ml

Tri-Color 6X DNA Loading Dye, 6 X 1ml

6000 applications

Protocol

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BIO-5170-1ml

FloroSafe DNA Stain, 1ml

~200 applications (based on precasting method)

Protocol

FAQ

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See also BUF - Product Listing for our Listing of popular buffers for DNA Electrophoresis.