High Performance Reagents for Routine PCR

Product Information
 
high_performance_reagents

Product No.

Product Description

Product Details

MSDS

BIO-5110-500U

Taq DNA Polymerase (recombinant), MgCl2separate, 500U

Supplied with 10x TaqBuffer and 25mM MgCl2

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BIO-5110-2500U

Taq DNA Polymerase (recombinant), MgCl2separate, 2500U

Supplied with 10x TaqBuffer and 25mM MgCl2

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BIO-5180-200 2X PCR Master Mix, 200 reactions Download Now Download Now
BIO-5180-1000 2X PCR Master Mix, 1000 reactions Download Now

BIO-5120-1ml

dNTP Mix, 10mM each, 1.0ml

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BIO-5125-1ml

dNTP Set, 100mM, 4 X 0.25ml

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C/No. BIO-5110-500U Taq DNA Polymerase (recombinant) - MgCl2 separate, 500U

Description

Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes 5'→3' synthesis of DNA, has zero detectable 3'→5' exonuclease (proofreading) activity and possesses minimal 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5kb or shorter.

Source

An E.coli strain that carries a cloned pol gene from Thermus aquaticus.

Unit Definition

One unit of the enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into a polynucleotide fraction in 30min at 74°C.

Applications

  • DNA sequencing
  • DNA labeling
  • PCR for cloning
  • PCR amplification of DNA fragments up to 5kb

Supplied with 10x Taq Buffer and 25mM MgCl2

Buffer Composition

Storage buffer

20mM Tris-HCl 0.1mM EDTA
0.5% (v/v) Nonidet P40 1mM DTT
0.5% (v/v) Tween 20 100mM KCl
50% (v/v) Glycerol  

10X Taq buffer without MgCl2
750mM Tris-HCl (pH 8.8 at 25°C)
200mM KCl
50mM (NH 4) 2SO 4
0.5% (v/v) Nonidet P40

Remarks

  • Half-life of this enzyme is >40mins @ 95°C.
  • Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as substrates for the DNA synthesis .

C/No. BIO-5120-1ml dNTP Mix, 10mM each

Description

Mixture contains aqueous solutions of the following at a final concentration of 10mM.

dATP
dTTP
dCTP
dGTP

With reduced number of pipetting steps, dNTPs remains highly pure which ensure successful PCR as the presence of contaminants can result in a decrease in amplification sensitivity and product yield.

Applications

  • DNA sequencing
  • DNA labeling
  • PCR
  • Long PCR
  • RT-PCR
  • cDNA synthesis
  • Primer extension

C/No. BIO-5125-1ml dNTP Set, 100mM each

Description

Contains 4 separate tubes of the following aqueous solutions at a concentration of 100mM each in 0.25ml.

2’-Deoxyadenosine-5’-triphosphate trisodium salt (dATP)
2’-Deoxythymidine-5’-triphosphate trisodium salt (dTTP)
2’-Deoxycytidine-5’-triphosphate trisodium salt (dCTP)
2’-Deoxyguanosine-5’-triphosphate trisodium salt (dGTP)

Applications

  • DNA sequencing
  • DNA labeling
  • PCR
  • Long PCR
  • RT-PCR
  • cDNA synthesis
  • Primer extension

Coloured PCR Reagents for Routine PCR

Product Information
 
high_performance_reagents

Product No.

Product Description

Product Details

MSDS

BIO-5115-500U

REDiant Taq DNA Polymerase (recombinant) - MgCl2separate, 500U

Supplied with 10x TaqBuffer and 25mM MgCl2

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BIO-5185-200

REDiant 2X PCR Master Mix, 200 reactions 

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BIO-5185-1000 REDiant 2X PCR Master Mix, 1000 reactions Download Now
- BIO-5186-200 exTEN 2X PCR Master Mix, 200 reactions Download Now Download Now
BIO-5186-1000 exTEN 2X PCR Master Mix, 1000 reactions Download Now