Chicken Polyclonal Antibody Packages Available

Two California Leghorn Chickens Standard PAb Packages

Service Name

Deliverables

Peptide Synthesis (15aa, > 70% purity)

Pre-immune Serum

Serum

Affinity-Purified Antibody

ELISA Report

PAb 8 week- CHI1 (2 Chicken)

Immunogen provided by researcher

Each chicken 1 mL (total 2 x 1mL)

Yes

Nil

Each Chicken On Bleed 1

PAb 8 week- CHI2 (2 Chicken)

5mg

PAb 8 week- CHI3 (2 Chicken)

Yes

2 x 12.5mL on Bleed 3

PAb 8 week- CHI4 (2 Chicken)

Yes

Total 200mL of serum

Standard 2 Chicken 8-week Anti-sera Production Schedule (Accelerated Schedule)

  • 8 week schedule
  • 2 chickens
  • 2 pre-bleeds
  • 8 immunizations
  • 6 production bleeds (1 mL each)
  • 3 egg collection periods 

Week

Procedure

0

Pre-immunization bleed: 
To collect 1mL of pre-immune serum (negative control) per chicken 

Primary immunization: 
Each chicken is injected with 100 µg of antigen emulsified in Complete Freund's Adjuvant (CFA)

2

Immunization No. 2:
50 µg antigen per chicken in Incomplete Freund's Adjuvant (IFA)

4

Immunization No. 3: 
50 µg antigen per chicken in Incomplete Freund's Adjuvant (IFA)

5

Production Bleed No. 1: 
1mL serum per chicken with ELISA Testing and Reporting

6

Immunization No. 4: 
50 µg antigen per chicken in Incomplete Freund's Adjuvant (IFA)

7

Production Bleed No. 2: 
1mL serum per chicken

8

Production Bleed No. 3: 
1mL serum per chicken (terminal bleed)